ANTIOXIDANT AND PHOTOPROTECTIVE POTENTIAL OF ETHYLACETATE FRACTION FROM ETHANOLIC EXTRACT OF RED DRAGON FRUIT PEEL (HYLOCERIUS POLYRHIZUS)

[ABSTRACT]Free radicals can be caused by exposure of UV rays. High free radicals in the body can be a trigger of many diseases. Photoprotectiveagent can protected the skin from exposure of UV rays. In addition, antioxidant compound can also reduced a negative impact of free radicals. Red dragon fruit (Hylocereuspolyrhizus) was one of the fruits which contain a flavonoid compound. This compound can acted as photoprotectiveand antioxidantagents. This study aims to know the content of flavonoid in the peel of red dragon fruit (Hylocereuspolyrhizus) and knowing the antioxidants and photoprotective activities. The peel of red dragon fruit (Hylocereuspolyrhizus) extracted within ethanol then macerate fractionated withethylacetate. The content of phenolic and flavonoid compounds of ethylacetate fraction of the red dragon fruit peel (RDFP-AcOEt) tested with TLC method, Folin- Ciocalteu method, and AlCl3 chelation method. Furthermore, the free radical (DPPH) scavenging test was to be done to found out the antioxidant activities from RDFP- AcOEt and in vitro test with spectrophotometry method was conducted to found out the SPF value of RDFP-AcOEt.The Total Phenolic Content ofRDFP-AcOEt was 1493.46 ± 14.97 mg GAE/100g and the Total Flavonoid Content ofRDFP-AcOEtwas 16.53 ± 0.36 % b/b EQ. Antioxidant activitiesofRDFP-AcOEt was weak (> 150 μg/mL). It was seen from the IC50 value of RDFP-AcOEt (491.94 μg/mL). Meanwhile,the SPF value ofRDFP-AcOEtwas very low (0.0069 ± 0.0071). So,the RDFP-AcOEt at a concentration of 5, 25, 50 and 100 mg/L does not have photoprotective activities.

https://opac.ll.chiba-u.jp/da/curator/107848/

https://opac.ll.chiba-u.jp/da/curator/107848/ceresIntlSympo-24-P270.pdf

Chiba University. Center for Environmental Remote Sensing

千葉大学環境リモートセンシング研究センター

会議発表用資料

Proceedings of the CEReS international symposium = CEReS国際シンポジウム資料集

24

270

279

2016-11-21

publisher

環境リモートセンシング研究センター「CEReSシンポジウム資料集」