STEROIDAL SAPONIN IN ETHANOL EXTRACT TUBER OF PURPLE YAM (Dioscorea alata L.) AS ALLERGENIC AGENT ON SENSITIVITY PHASE BALB/c MICE MODEL ALLERGY

[ABSTRACT]Purple yam (Dioscorea alata L.) is a source of biological tubers not been used optimally. Diosgenin is steroids saponin compounds that most important because it has multiple biological functions, such as allergenic activity. The objective of this research was to analyze the potential allergenic ethanol extract tuber of D. alata L. (EEDA) on BALB/c mice on sensitivity phase with measure the profile of transcription factor FoxP3 Treg cells and cytokine profile of Treg and IgE and IgG1 B cells. An experimental study using BALB/c mice divided into 7 groups: control group (I), the treatment group (II-V) ethanol extract of tuber Dioscorea alata L. dose of 0.00; 0.17; 2.01; 10.04 g/kg bw, the treatment group antihistamines drug and Diosgenin (VI-VII). For 17 consecutive days the group II-VII were treated in accordance with the group and with Ovalbumin induced allergy models. Mice were sacrificed on day 18. Spleen is removed, lymphocyte isolated and analyze the transcription factor FoxP3 Treg cells, cytokine profile of T reg cells and IgE and IgG1 B cells on spleen using Flowcytometry FACS Calibur. The results showed EEDA able to inhibit the production of B220IgE and B220IgG1, trigger Treg cells (CD4CD25) and the transcription factor FoxP3 (CD4CD25FoxP3) and profile of the cytokines produced by T reg cells. The conclusion is ethanol extract tubers of Dioscorea alata L (EEDA) does not trigger Treg (CD4CD25) and the transcription factor FoxP3 (CD4CD25FoxP3) and cytokine profiles produced by Treg cells namely CD4IL-10, CD8IL-10, CD4TGF-β and CD8TGF-β in the sensitivity phase.

https://opac.ll.chiba-u.jp/da/curator/107836/

https://opac.ll.chiba-u.jp/da/curator/107836/ceresIntlSympo-24-P175.pdf

Chiba University. Center for Environmental Remote Sensing

千葉大学環境リモートセンシング研究センター

Presentation

Proceedings of the CEReS international symposium = CEReS国際シンポジウム資料集

24

175

184

2016-11-21

eng

publisher

Proceedings of the symposium on environmental remote sensing