Substrate utilization and extracellular enzymes of Agaricus bisporus

Wood, David Arthur

Agaricus bisporus

研究概要:The white mushroom, Agaricus bisporus, is cultivated on a straw based composted substrate. This basidiomycete fungus utilises for growth both plant and microbial biopolymers during colonisation of its commercial growth medium, composted wheat straw. The polymers utilised by the mycelium include cellulose and hemicelluloses, from plant cell walls, peptidoglycans and glucans from microbial cell walls and protein from both sources. The possible utilisation of lignin or lignin derived polymers is not yet confirmed although the fungus possesses the necessary enzyme systems for lignin degradation. Bioconversion of these polymers is effected by a set of extracellular enzymes produced by the mycelium of the fungus. Several of the enzymes responsible for substrate bioconversion have been characterised and some cases purified and antibodies produced against the pure proteins. This has lead to the expression cloning of the structural genes and identification of their upstream sequences which regulate the transcriptional level of extracellular enzyme secretion. The activities of laccase and cellulase are strongly regulated during the cycles of mushroom production and this regulation is associated with genetic regulation at the transcriptional level and with protein biosynthesis."

https://opac.ll.chiba-u.jp/da/curator/900021293/

https://opac.ll.chiba-u.jp/da/curator/900021293/M-10_15.pdf

千葉大学真菌医学研究センター

研究報告書

千葉大学真菌医学研究センター報告

2

69

74

1998

真菌医学研究センター報告