YONEYAMA, Shingo

The effectiveness of a manure consisting of domestic fowl droppings, rice bran and some unknown fungi and other microorganisms in controlling nematodes has been known in practice. It was found that bouillon extract of this manure shows nematicidal and ovicidal actions against Rhabditis, Meloidogyne, in vitro. We have isolated several bacteria and fungi from this bouillon extract. Most of this bacteria belonged Genus Pseudomonas, while one of the fungi was found to be Candida pellicurosa. Pure bouillon cultures of these bacteria killed, in immersion tests, Rhabditis, Panagrellus, Meloidogyne. Some of these bacteria also showed liquefying actions on Rhabditis. The bouillon cultures were filtered through a Seitz's filter. It was found that these filtrates also have nematicidal as well as nema-liquefying actions, also fungicidal action against Corticium rolfsii (sclerotium), and Fusarium oxysporum f. niveum and Alternaria kikuchiana (conidia). The cause of this nematicidal and fungicidal actions which may be an antibiotic substance, a proteolytic enzyme, or a phage, must be left to further investigations.