Studies on the Anaerobic Cellulolytic Bacteria : Report 1. Isolation and Classification of the Anaerobic Cellulolytic Bacteria

In reference to the studies of the procedure of human waste in our laboratory, I have investigated the anaerobic cellulolytic bacteria, on supposition that these bacteria liquefy paper and cellulose substances in human waste. We have been much interested on the problem as to reducing of scum and sediment in accumulated excretions. The author has isolated 51 strains of anaerobic cellulolytic bacteria from sludge of pons and feces of horses etc., and divided them into 3 varieties. ( group K_1, K_2, K_3) Main physiological characters of the bacteria are as follows. 1) Bacteria of group Kt are rods, 2.4 to 6.0 by 0.4 microns, longitudinal curving, occurring singly, no chains. Spores ovale (1.4 by 1.0), terminal, swelling rods. Gram negative, nonmotile. K_2 are rods, 2.0 to 7.0 by 0.4 to 0.7 microns, longitudinale curving, occurring singly, no chains. Spores ovale (2.0 by 1.4), terminal, swelling rods. Gram negative, non-motile. K_3 are rods, 0.7 to 1.0 by 0.4 microns, occurring singly, no chains. Spores ovale (0.6 by 0.4), terminal. Gram negative, non-motile. 2) Cellulose agar deep colonies. (1.2 -0x1.f6620bfff517p+0gar) grayish white, delicate, cottony, filmy, scarcely discernible, the transparent ring for the cellulolyse around the colonies. 3) Cellulose agar surface colonies. (1.2 0x1.5666572206e49p+807gar) grayish white, delicate, filmy, rand irregular, the transparent ring for the cellulolyse around the colonies, smell butyricacid. Cellulose agar surface colonies. (2 0x1.f742065636e65p-505gar) grayish white, little, hemispheric, glossily, the transparent ring for the cellulolyse around the colonies, smell butyricacid. 4) Cellulose nutrient solution. Cellulose is destroyed in 7-14 days and gas bubbles arise. pH of the solution varies from 7.5 to 6.0. 5) Broth. K_1, K_2 : no growth. K_3 : growth and slight sediment, but the solution remains visibly clear. 6) Glucose broth. K_1 : no growth. K_2, K_3 : growth and slight sediment, but the solution remains visibly clear.