高橋, 幸枝

Takahashi, Yukie

Although the plants that belong to Genus Elaeagnus are non-leguminous, they have conspicuous root nodules. This report deals with the isolation of the endophyte which yields root nodules on the roots of the three plants : Elaeagnus macrophylla, Elaeagnus glabra and Elaeagnus pungens. By using the root nodules of and the soil around the roots of these plants as the materials for the experiments, the present author has succeeded in the isolation and pure culture of two strains of Actinomycetes common to each other. From the morphological and cultural aspects, one of them was identified as Streptomyces violascens (Preobrazhenskaya and Sveshnikova) Pridham et al. 1958, 68. ISP 5183 but the other was diagnosed as Streptomyces sp. because it did not indicate any morphological and cultural characteristics which are necessary for its identification. Therefore, about the latter, more of the data are now under examination. The filtrate of the liquid medium in which Streptmyces violascens was cultured, was proved as antibiotic to Bacillus subtilis only. Considering the results of the experiments, it is supposed that Actinomycetes producing the root nodules on the roots of the plants that belong to genus Elaeagnus may originate in the soil around their roots.